From: Kenny Nam on 4 Aug 2010 02:34 "Daphne" <daphnew_too_nospam(a)yahoo.com> wrote in message <hudrc3$2oi$1(a)fred.mathworks.com>... > > Thanks so much! > Your demo and the histogram codes were exactly what I needed. > Tinkered with the images a bit and found the threshold range for yellow. > > Thanks again! > Daphne Daphne,i also doing the yellow color detection,do u filter the background of the picture before you get the range for yellow?Can share your idea to me?
From: Daphne on 4 Aug 2010 10:12 Hi Kenny, Any filtration or brightness/contrast is typically set by the user when taking the image and we count on the quality of the staining. We filter objects for brightness and size. I upload two images into the red and the green, use imadd to get the merged image and then define the color mask to get only the yellow (got the yellow limits or its RGB definition from a spectrum plot). Then I filter a bit more by size of blob, not to get background stains of single pixels etc. Hope this helps, Daphne "Kenny Nam" <kennynam517(a)hotmail.com> wrote in message <i3b1l3$3a4$1(a)fred.mathworks.com>... > "Daphne" <daphnew_too_nospam(a)yahoo.com> wrote in message <hudrc3$2oi$1(a)fred.mathworks.com>... > > > > Thanks so much! > > Your demo and the histogram codes were exactly what I needed. > > Tinkered with the images a bit and found the threshold range for yellow. > > > > Thanks again! > > Daphne > > Daphne,i also doing the yellow color detection,do u filter the background of the picture before you get the range for yellow?Can share your idea to me?
From: Kenny Nam on 9 Aug 2010 12:01 "Daphne" <daphnew_too_nospam(a)yahoo.com> wrote in message <i3bsfi$fu1$1(a)fred.mathworks.com>... > Hi Kenny, > > Any filtration or brightness/contrast is typically set by the user when taking the image and we count on the quality of the staining. We filter objects for brightness and size. > > I upload two images into the red and the green, use imadd to get the merged image and then define the color mask to get only the yellow (got the yellow limits or its RGB definition from a spectrum plot). Then I filter a bit more by size of blob, not to get background stains of single pixels etc. > > Hope this helps, > Daphne > > > "Kenny Nam" <kennynam517(a)hotmail.com> wrote in message <i3b1l3$3a4$1(a)fred.mathworks.com>... > > "Daphne" <daphnew_too_nospam(a)yahoo.com> wrote in message <hudrc3$2oi$1(a)fred.mathworks.com>... > > > > > > Thanks so much! > > > Your demo and the histogram codes were exactly what I needed. > > > Tinkered with the images a bit and found the threshold range for yellow. > > > > > > Thanks again! > > > Daphne > > Nam Kenny > > Daphne,i also doing the yellow color detection,do u filter the background of the picture before you get the range for yellow?Can share your idea to me? Daphne, I have try to adjust the threshold for the demo of simple color detection,but it not work,it still show the histogram in RGB.Do u mind to show me your coding in detect or filter the yellow?Need your help/guide badly.Thanks
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