From: ImageAnalyst on
Look at the blue channel. Your inner blob is fairly distinct there
with a threshold of 228. However your outer blob has a very poorly
defined outer border that connects it to the bright stuff at the edge
of the image.

You really need to work on getting a better image to start with.
Better illumination, better contrast, no letters written on your
sample, etc.
From: Beho nashed on
ImageAnalyst <imageanalyst(a)mailinator.com> wrote in message <3a1a72c4-5c73-4949-b1f9-077741af3dc3(a)z31g2000vbk.googlegroups.com>...
> Look at the blue channel. Your inner blob is fairly distinct there
> with a threshold of 228. However your outer blob has a very poorly
> defined outer border that connects it to the bright stuff at the edge
> of the image.
>
> You really need to work on getting a better image to start with.
> Better illumination, better contrast, no letters written on your
> sample, etc.

I will give it another try. I have the picture at 5 MP, but I had to adjust it to 1 MP , as matlab kept freezing and crashing.

I have another question, Is there away to change the yellow color in the image generated by imadjust to black?
Thanks for your help.
From: ImageAnalyst on
On Jun 16, 7:29 pm, "Beho nashed" <beh...(a)yahoo.com> wrote:
> I have another question, Is there away to change the yellow color in the image generated by imadjust to black?
> Thanks for your help.
-------------------------------------------------------------------------
Sure, but you shouldn't even be using imadjust.

From: Beho nashed on
ImageAnalyst <imageanalyst(a)mailinator.com> wrote in message <5b824ca7-29cd-4811-b9ef-013857810b01(a)g19g2000yqc.googlegroups.com>...
> On Jun 16, 7:29 pm, "Beho nashed" <beh...(a)yahoo.com> wrote:
> > I have another question, Is there away to change the yellow color in the image generated by imadjust to black?
> > Thanks for your help.
> -------------------------------------------------------------------------
> Sure, but you shouldn't even be using imadjust.

Hello ImageAnalyst,
I took more pics of the sample. for the text, It's already there!!! I tried to remove it using GIMP software, it's not that bad.

I uploaded the new pic here --> http://drop.io/rlv072i (3rd pic)

I am trying to calculate the large area. how would you approach it?
From: ImageAnalyst on
I know the text (letters) are already there. Can you get rid of
them? Snap a photo without them? Also, even with your latest photo,
it's very unclear where you consider the boundaries of the object to
be.