From: David Young on
> It is the blood vessels(not nerves). The blood vessels can be extracted by using a method called as the Kirch's method. But, my aim is to confirm if the image has the blood vessels. say that i have detected the blood vessels- how to confirm this detection other that visually inspecting the image(this is important for the later stages of the algorithm).
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It's Kirsch's method (with an s).

I'm afraid I don't understand what you mean by "confirming the detection". Would applying a different detection method and comparing the results be one way?

Also, your original post asked for a detection method. It would be helpful if you'd shared your awareness of Kirsch's method at that point and explained what you really needed.
From: ImageAnalyst on
The only nerves visible are where they are all bundled together at the
optic nerve and head off to the brain. Elsewhere they are invisible.
The meandering things you see are blood vessels. By the way, an
ophthalmologist I used to work with said that 42% of all nerves in the
human body are in the retinas. I thought that was a surprisingly high
number, but once I thought about it it seemed understandable.

Have you looked at other methods people have successfully used, too
numerous to list here but listed here:
http://iris.usc.edu/Vision-Notes/bibliography/medical853.html#Retinal%20Images,%20Analysis%20of%20Eye,%20etc.