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From: Azy on 19 Jul 2010 05:30
Hi ..Actlly my intrest in image processing has just started growing thru matlab..
I have take an image which contains cells woth nuclei inside. It was a color image so i converted it to gray scale image. I find it hard to process ,so i converted it to binary image. NOw after connverting it binary image, im not able to plot boundaries of it properly as compared to ur coins boundary tracing example. can u help me out plz.

My aim is to sort out the round cells in image and count them.
From: Sean on 19 Jul 2010 10:08
"Azy " <azhar421(a)yahoo.com> wrote in message <i215vd$j32$1(a)fred.mathworks.com>...
> Hi ..Actlly my intrest in image processing has just started growing thru matlab..
> I have take an image which contains cells woth nuclei inside. It was a color image so i converted it to gray scale image. I find it hard to process ,so i converted it to binary image. NOw after connverting it binary image, im not able to plot boundaries of it properly as compared to ur coins boundary tracing example. can u help me out plz.
>
> My aim is to sort out the round cells in image and count them.

Can you show us your images?
Take a look at:
>>doc bwboundaries
>>doc edge

If you want to measure "circularity" one easy way would be to take the (actual area)/(pi*equivdiameter/4) which would measure the the shape compared to an ideal circle. Both actual area and equivdiameter are outputs of regionprops().
From: Azy on 26 Jul 2010 04:46
"Sean " <sean.dewolski(a)nospamplease.umit.maine.edu> wrote in message <i21m84$t1$1(a)fred.mathworks.com>...
> "Azy " <azhar421(a)yahoo.com> wrote in message <i215vd$j32$1(a)fred.mathworks.com>...
> > Hi ..Actlly my intrest in image processing has just started growing thru matlab..
> > I have take an image which contains cells woth nuclei inside. It was a color image so i converted it to gray scale image. I find it hard to process ,so i converted it to binary image. NOw after connverting it binary image, im not able to plot boundaries of it properly as compared to ur coins boundary tracing example. can u help me out plz.
> >
> > My aim is to sort out the round cells in image and count them.
>
> Can you show us your images?
> Take a look at:
> >>doc bwboundaries
> >>doc edge
>
> If you want to measure "circularity" one easy way would be to take the (actual area)/(pi*equivdiameter/4) which would measure the the shape compared to an ideal circle. Both actual area and equivdiameter are outputs of regionprops().



Thanks for showing your interest:

My image is : http://i29.tinypic.com/4qt1tk.jpg

Its a color image . It has got a cirular red blood cells and a big white cell in a different color. I want to count those red cells excluding all other white cells.
From: ImageAnalyst on 9 Aug 2010 12:41
I think Sean meant pi*equivdiameter^2/4
From: Sean on 9 Aug 2010 12:52
ImageAnalyst <imageanalyst(a)mailinator.com> wrote in message <35aed27e-5ee4-4ed4-8bd7-cf01bc8e5574(a)q22g2000yqm.googlegroups.com>...
> I think Sean meant pi*equivdiameter^2/4

Good catch!
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